Белки и пептиды в постгеномную эру. Структурно-функциональные исследования для решения фундаментальных задач и направленного конструирования инновационных лекарственных средств
Интенсивные структурные исследования геномов эукариотических и прокариотических организмов, населяющих нашу планету, обусловили новый этап в развитии биологии и ее прикладных направлений - биотехнологии, биомедицины, фармацевтики и других. В настоящее время кажется, что функциональная геномика (транскриптомика) способна дать ответы на новые вызовы современности: указать, какие механизмы обуславливают развитие заболеваний человека и животных, каталогизировать генетические вариации, наличие которых ассоциировано с рисками развития недугов человека и, наконец, разработать технологии коррекции на генетическом уровне «ошибок» природы, наличие которых обуславливает болезни. Однако при ближайшем рассмотрении становится понятно, что в гораздо большей степени современное человечество возлагает надежды на использование других составляющих живой системы - белков и пептидов. Бурный всплеск технологий манипулирования нуклеиновыми кислотами обусловил интенсивное развитие производства диагностических и терапевтических антител для ранней диагностики и эффективного таргетного лечения. Фундаментальные исследования взаимосвязи структуры и функции белков и пептидов играют ведущую роль в настоящее время и будут играть всё большую роль в будущих периодах, так как именно белково-пептидные вещества выполняют основные биологические функции в каждой живой клетке и в организме в целом. Значительный прогресс в белково-пептидной химии был определен внедрением в исследовательскую практику современного масс-спектрометрического анализа и других физико-химических методов, таких как ЯМР-спектроскопия и рентгеноструктурный анализ. Современная наука, занимающаяся изучением белков и пептидов, представляет собой комплексную дисциплину, использующую все современные методы и технологии: приёмы генной инженерии, методы структурной и квантовой химии, походы высокопроизводительного масс-спектрометрического анализа, позволяющие идентифицировать и количественно определять многие тысячи белков и пептидов, создавая их молекулярный каталог со свойственными энзиматическими модификациями, происходящим с белками и пептидами после их синтеза в клетках. Разработка новых технологий исследования белков и пептидов, изучения их посттрансляционных модификаций, количественного определения этих компонентов в биологических объектах, использования их рационального компьютерного дизайна – вот далеко не полный перечень актуальных направлений развития этой приоритетной области знания, определяющей успех современной фармацевтики и персонифицированной медицины в недалеком будущем. Институт биоорганической химии им. академиков М.М.Шемякина и Ю.А.Овчинникова Российской академии наук (ИБХ РАН) является головным учреждением по изучению белков и пептидов в нашей стране. За время существования ИБХ РАН фундаментальные исследования в области белково-пептидной химии снискали международный авторитет и позволили разработать и внедрить в практику белковые и пептидные препараты как в нашей стране, так и за её пределами. На новом этапе своей деятельности мы предлагаем комплексный проект, который состоит из пяти неразрывно связанных с друг другом блоков и охватывает все основные направления современной науки, занимающейся изучением взаимосвязи структуры и функции белков и пептидов. Одним из важных критериев такого исследования будет являться наличие как современных конкурентоспособных технологий анализа полипептидов (масс-спектрометрия, ЯМР-спектроскопия, высокоэффективная хроматография, гетерологичная экспрессия с использованием новых векторных систем, создание трансгенных нокаутных экспериментальных животных и др.), так фундаментальных исследований в области пептидомики и физиологии рецепторов, способных выявить новые функции этого класса природных соединений и положить начало конструированию инновационных лекарственных средств. Наряду с этим, особое внимание будет уделено вопросам обратной генетики, в частности, идентификации пептидов, кодируемых короткими рамками считывания у растений, а также разработке новых алгоритмов для поиска и идентификации пептидов, кодирующихся участками длинных некодирующих РНК. Эти примеры еще раз показывают, что тесное взаимодействие некогда отдельных дисциплин является не только источником и критерием достоверности результатов фундаментальных научных исследований, но и существенно ускоряет их внедрение в практику.
6 Января 2014 года — 31 Декабря 2018 года
Список публикаций по проекту
- (2016). A novel lipid transfer protein from the dill Anethum graveolens L.: Isolation, structure, heterologous expression, and functional characteristics. J Pept Sci 22 (1), 59–66
- (2017). Improved strategy for recombinant production and purification of antimicrobial peptide tachyplesin I and its analogs with high cell selectivity. J Appl Biochem 64 (1), 35–42
- (2016). Effective lipid-detergent system for study of membrane active peptides in fluid liposomes. J Pept Sci 22 (2), 98–105
- (2019). Release of Porcine Sperm from Oviduct Cells is Stimulated by Progesterone and Requires CatSper. Sci Rep 9 (1), 19546
- (2020). Are there specific antibodies against Neu5Gc epitopes in the blood of healthy individuals? Glycobiology 30 (6), 395–406
- (2019). SOX9 as One of the Central Units of Regulation Axis of Pancreas Embryogenesis and Cancer Progression. Mol. Gen. Microbiol. Virol. 34 (3), 159–169
- (2020). Structure of Supramers Formed by the Amphiphile Biotin-CMG-DOPE. ChemistryOpen 9 (6), 641–648
- (2020). Human Natural Antibodies Recognizing Glycan Galβ1-3GlcNAc (LeC). Int J Mol Sci 21 (18), 1–15
- (2020). Specificity of human natural antibodies referred to as anti-Tn. Mol Immunol 120, 74–82
- (2019). Activation of IGF/IGF-IR signaling pathway fails to induce epithelial-mesenchymal transition in pancreatic cancer cells. Pancreatology 19 (2), 390–396
- (2019). Printed Glycan Array: A Sensitive Technique for the Analysis of the Repertoire of Circulating Anti-carbohydrate Antibodies in Small Animals. J Vis Exp 2019 (144),
- (2019). Modeling of Phosphoribosylpyrophosphate Synthetase from Thermus Thermophilus in Complex with ATP and Ribose 5-Phosphate. Cryst. Rep 64 (1), 94–97
- (2019). Study of the Strain Bacillus pumilus B-13176 and Its Metabolites with Fungicidal and Antibacterial Activities against Aspergillus niger and Staphylococcus aureus (MRSA). APPL BIOCHEM MICRO+ 55 (7), 748–755
- (2018). Therapy-induced stress response is associated with downregulation of pre-mRNA splicing in cancer cells. Genome Med 10 (1), 49
- (2021). Specificity profile of αGal antibodies in αGalT KO mice as probed with comprehensive printed glycan array: Comparison with human anti‐Galili antibodies. Xenotransplantation 28 (3), e12672
- (2021). IgA1 Protease as a Vaccine Basis for Prevention of Bacterial Meningitis. Russ. J. Bioorganic Chem. 47 (4), 805–814
- (2019). The architecture of the IgG anti-carbohydrate repertoire in primary antibody deficiencies. Blood 134 (22), 1941–1950
- (2023). Antibodies against hyaluronan oligosaccharides in xenotransplantation. Xenotransplantation 30 (3), e12799
- (2015). Truncated variants of Serratia proteamaculans oligopeptidase B having different activities. Biochemistry (Mosc) 80 (10), 1331–1343
- (2016). Novel fluorescent membrane probe 2,3;5,6-bis(cyclohexyl)-BODIPY-labeled phosphatidylcholine. Russ. J. Bioorganic Chem. 42 (3), 305–309
- (2015). Сравнительная оценка мурамил пептидов – лигандов рецепторов врожденного иммунитета в регуляции экспрессии оксида азота. (4), 453–456
- (2016). A Cascade of Thermophilic Enzymes As an Approach to the Synthesis of Modified Nucleotides. Acta Naturae 8 (4), 82–90
- (2016). Scope and limitations of MALDI-TOF MS blood serum peptide profiling in cancer diagnostics. Russ. J. Bioorganic Chem. 42 (5), 497–505
- (2016). The interaction of secreted proteins Noggin4 and Wnt8 from Xenopus laevis embryos. Russ. J. Bioorganic Chem. 42 (3), 340–342
- (2017). Antimicrobial activity of endogenous peptides of the moss Physcomitrella patens. Russ. J. Bioorganic Chem. 43 (3), 248–254
- (2016). Expression Of DNA-Encoded Antidote to Organophosphorus Toxins in the Methylotrophic Yeast Pichia Pastoris. APPL BIOCHEM MICRO+ 52 (2), 184–192
- (2016). Antimicrobial peptides of invertebrates. Part 1. structure, biosynthesis, and evolution. Russ. J. Bioorganic Chem. 42 (3), 229–248
- (2016). Antimicrobial peptides of invertebrates. Part 2. biological functions and mechanisms of action. Russ. J. Bioorganic Chem. 42 (4), 343–360
- (2015). Cancer stem cells: Plasticity works against therapy. Acta Naturae 7 (4), 46–55
- (2015). Are super-enhancers regulators of regulatory genes of development and cancer? Mol Biol 49 (6), 818–824
- (2015). Master transcription regulators specifying cell-lineage fates in development as possible therapeutic targets in oncology. Russ J Genet 51 (11), 1049–1059
- (2017). The secreted protein Noggin4 is an activator of the Wnt/PCP-signaling pathway. Russ. J. Bioorganic Chem. 43 (2), 216–219
- (2016). Biofunctionalizing nanofibers with carbohydrate blood group antigens. Biopolymers 105 (11), 787–794
- (2016). Photoinduced Changes of Surface Topography in Amorphous, Liquid-Crystalline, and Crystalline Films of Bent-Core Azobenzene-Containing Substance. J Phys Chem B 120 (22), 5073–5082
- (2016). Quantum dot–polymer composites based on nanoporous polypropylene films with different draw ratios. Eur Polym J 82, 93–101
- (2016). A novel lipid transfer protein from the pea Pisum sativum: Isolation, recombinant expression, solution structure, antifungal activity, lipid binding, and allergenic roperties. BMC Plant Biol 16 (1), 107
- (2017). Novel Thionins from Black Seed (Nigella sativa L.) Demonstrate Antimicrobial Activity. Int J Pept Res Ther 23 (2), 171–180
- (2016). Studying of cellular interaction of hairpin-like peptide EcAMP1 from barnyard grass (Echinochloa crusgalli L.) seeds with plant pathogenic fungus Fusarium solani using microscopy techniques. Scanning 38 (6), 591–598
- (2016). Ultra-fast glyco-coating of non-biological surfaces. Int J Mol Sci 17 (1),
- (2016). Recombinant phosphoribosyl pyrophosphate synthetases from Thermus thermophilus HB27: Isolation and properties. Russ. J. Bioorganic Chem. 42 (5), 512–521
- (2016). Chemical Platform for the Preparation of Synthetic Orally Active Peptidomimetics with Hemoregulating Activity. ChemMedChem 11 (18), 1974–1977
- (2016). The pathogenesis of the demyelinating form of guillain-barre syndrome (GBS): Proteopeptidomic and immunological profiling of physiological fluids. Mol Cell Proteomics 15 (7), 2366–2378
- (2016). Chicken GRIFIN: A homodimeric member of the galectin network with canonical properties and a unique expression profile. Biochimie 128129, 34–47
- (2016). Galectin-related protein: An integral member of the network of chicken galectins 1. From strong sequence conservation of the gene confined to vertebrates to biochemical characteristics of the chicken protein and its crystal structure. BIOCHIM BIOPHYS ACTA 1860 (10), 2285–2297
- (2016). Downregulation of expression of mater genes SOX9, FOXA2, and GATA4 in pancreatic cancer cells stimulated with TGFβ1 epithelial–mesenchymal transition. Dokl Biochem Biophys 469 (1), 257–259
- (2016). Muramyl peptides activate innate immunity conjointly via YB1 and NOD2. Innate Immun 22 (8), 666–673
- (2016). Serological Analysis of Immunogenic Properties of Recombinant Meningococcus IgA1 Protease-Based Proteins. Bull Exp Biol Med 161 (3), 391–394
- (2016). Structural basis of p75 transmembrane domain dimerization. J Biol Chem 291 (23), 12346–12357
- (2016). The effect of silver nanoparticles on the photocycle of bacteriorhodopsin of purple membranes of Halobacterium salinarum. Opt Spectrosc 121 (2), 210–219
- (2016). Stereo- and regioselective synthesis of spacer armed α2-6 sialooligosaccharides. MENDELEEV COMMUN 26 (5), 380–382
- (2017). Dimerization of the antimicrobial peptide arenicin plays a key role in the cytotoxicity but not in the antibacterial activity. Biochem Biophys Res Commun 482 (4), 1320–1326
- (2016). Blood plasma-derived anti-glycan antibodies to sialylated and sulfated glycans identify ovarian cancer patients. PLoS One 11 (10), e0164230
- (2016). Block synthesis of A (type 2) and B (type 2) tetrasaccharides related to the human ABO blood group system. Carbohydr Res 430, 59–71
- (2016). Function-spacer-lipid constructs of Lewis and chimeric Lewis/ABH glycans. Synthesis and use in serological studies. Carbohydr Res 435, 83–96
- (конференция) (2015). Submicron QDs-containing Particles as Nano-thermosensors. Mater Today 3 (2), 617–621
- (2016). Comprehensive analysis of draft genomes of two closely related pseudomonas syringae phylogroup 2b strains infecting mono- and dicotyledon host plants. BMC Genomics 17 (Suppl 14), 1010
- (2016). The Physcomitrella patens chloroplast proteome changes in response to protoplastation. Front Plant Sci 7 (2016), 1661
- (книга) (2018). Analysis of endogenous peptide pools of Physcomitrella patens moss. Methods Mol Biol 1719, 395–405
- (2016). The cause of cancer mutations: Improvable bad life or inevitable stochastic replication errors? Mol Biol 50 (6), 799–811
- (2016). Fundamentally low reproducibility in molecular genetic cancer research. Russ J Genet 52 (7), 650–663
- (2016). KLF5, a new player and new target in the permanently changing set of pancreatic cancer molecular drivers. Russ. J. Bioorganic Chem. 42 (6), 606–611
- (2016). 1001 lights: Luciferins, luciferases, their mechanisms of action and applications in chemical analysis, biology and medicine. Chem Soc Rev 45 (21), 6048–6077
- (2016). Properties, functions, and therapeutic prospects of enhancer RNAs. Russ. J. Bioorganic Chem. 42 (5), 473–478
- (2016). Multidimensional complexity of cancer. Simple solutions are needed. Biochemistry (Mosc) 81 (7), 731–738
- (2017). Plant pathogenesis-related proteins PR-10 and PR-14 as components of innate immunity system and ubiquitous allergens. Curr Med Chem 24 (17), 1772–1787
- (2017). Selected Least Studied but not Forgotten Bioluminescent Systems. Photochem Photobiol 93 (2), 405–415
- (2017). Expression of transcription factor genes in cell lines corresponding to different stages of pancreatic cancer progression. Dokl Biochem Biophys 475 (1), 267–270
- (2017). Pore formation in lipid membrane II: Energy landscape under external stress. Sci Rep 7 (1), 12509
- (2017). Altered (neo-) lacto series glycolipid biosynthesis impairs α2-6 sialylation on N-glycoproteins in ovarian cancer cells. Sci Rep 7, 45367
- (2017). Presence of homeobox gene of Anf class in Pacific lamprey Lethenteron camtschaticum confirms the hypothesis about the importance of emergence of Anf genes for the origin of telencephalon in vertebrate evolution. RUSS J DEV BIOL 48 (4), 241–251
- (2017). Repertoire of BALB/c mice natural anti-carbohydrate antibodies: Mice vs. humans difference, and otherness of individual animals. Front Immunol 8 (NOV), 1449
- (2017). Network inference from glycoproteomics data reveals new reactions in the IgG glycosylation pathway. Nat Commun 8 (1), 1483
- (2017). The Conformation of the Epidermal Growth Factor Receptor Transmembrane Domain Dimer Dynamically Adapts to the Local Membrane Environment. Biochemistry 56 (12), 1697–1705
- (2017). Conformational transitions and interactions underlying the function of membrane embedded receptor protein kinases. BIOCHIM BIOPHYS ACTA 1859 (9), 1417–1429
- (2017). Bioluminescence: Is it possible for a plant? Bulletin of Russian State Medical University 6 (2), 56–65
- (2017). Alkaline pH induces IRR-mediated phosphorylation of IRS-1 and actin cytoskeleton remodeling in a pancreatic beta cell line. Biochimie 138, 62–69
- (2017). Impact of membrane partitioning on the spatial structure of an S-type cobra cytotoxin. J Biomol Struct Dyn 36 (13), 1–16
- (2017). Evolution of inhibitor-resistant natural mutant forms of HIV-1 protease probed by pre-steady state kinetic analysis. Biochimie 142, 125–134
- (2018). Immunogenic and Protective Properties of Neisseria meningitidis IgA1 Protease and of Its Truncated Fragments. Russ. J. Bioorganic Chem. 44 (1), 64–72
- (2017). Construction of a combinatorial library of chimeric tumor-specific promoters. Biotechniques 63 (3), 107–116
- (2017). Expression of master regulatory genes of embryonic development in pancreatic tumors. Dokl Biochem Biophys 475 (1), 250–252
- (2017). Dependence of expression of regulatory master genes of embryonic development in pancreatic cancer cells on the intracellular concentration of the master regulator PDX1. Dokl Biochem Biophys 475 (1), 259–263
- (2017). Cardiotoxins: Functional Role of Local Conformational Changes. J Chem Inf Model 57 (11), 2799–2810
- (2017). Comparative in vitro study on cytotoxicity of recombinant β-hairpin peptides. Chem Biol Drug Des 91 (1), 294–303
- (2017). Activity modulation of the oligopeptidase B from Serratia proteamaculans by site-directed mutagenesis of amino acid residues surrounding catalytic triad histidine. Biochimie 139, 125–136
- (2017). Identification of hispidin as a bioluminescent active compound and its recycling biosynthesis in the luminous fungal fruiting body. Photochem Photobiol Sci 16 (9), 1435–1440
- (2017). A therapeutic potential of animal β-hairpin Antimicrobial Peptides. Curr Med Chem 24 (17), 1724–1746
- (2017). Fluorescent protein Dendra2 as a ratiometric genetically encoded pH-sensor. Biochem Biophys Res Commun 493 (4), 1518–1521
- (2017). Crystallization and preliminary X-ray diffraction study of recombinant adenine phosphoribosyltransferase from the thermophilic bacterium Thermus thermophilus strain HB27. Cryst. Rep 62 (4), 580–583
- (2017). Structural study of the β-hairpin marine antimicrobial peptide arenicin-2 in PC/PG lipid bilayers by fourier transform infrared spectroscopy. Russ. J. Bioorganic Chem. 43 (5), 502–508
- (2018). Marine antimicrobial peptide arenicin adopts a monomeric twisted β-hairpin structure and forms low conductivity pores in zwitterionic lipid bilayers. Peptide Science 110 (2),
- (2017). Crystal structure of recombinant phosphoribosylpyrophosphate synthetase 2 from Thermus thermophilus HB27 complexed with ADP and sulfate ions. Acta Crystallogr F Struct Biol Commun 73 (6), 369–375
- (2017). Gram scale synthesis of A (type 2) and B (type 2) blood group tetrasaccharides through 1,6-anhydro-N-acetyl-β-D-glucosamine. Carbohydr Res 449, 65–84
- (2017). Alternative splicing shapes transcriptome but not proteome diversity in Physcomitrella patens. Sci Rep 7 (1), 2698
- (2017). Unveiling Structural Motions of a Highly Fluorescent Superphotoacid by Locking and Fluorinating the GFP Chromophore in Solution. J Phys Chem Lett 8 (23), 5921–5928
- (2017). PDX1: A unique pancreatic master regulator constantly changes its functions during embryonic development and progression of pancreatic cancer. Biochemistry (Mosc) 82 (8), 887–893
- (2017). The role of the signaling pathway FGF/FGFR in pancreatic cancer. Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry 11 (2), 101–110
- (2018). The Role of Intercellular Communication in Cancer Progression. Russ. J. Bioorganic Chem. 44 (5), 473–480
- (2018). Peptidomics dataset: Blood plasma and serum samples of healthy donors fractionated on a set of chromatography sorbents. Data Brief 18, 1204–1211
- (2018). LogLoss-BERAF: An ensemble-based machine learning model for constructing highly accurate diagnostic sets of methylation sites accounting for heterogeneity in prostate cancer. PLoS One 13 (11), e0204371
- (2018). Histo-blood group antigen-binding specificities of human rotaviruses are associated with gastroenteritis but not with in vitro infection. Sci Rep 8 (1), 12961
- (2018). Structural basis of the signal transduction via transmembrane domain of the human growth hormone receptor. BIOCHIM BIOPHYS ACTA 1862 (6), 1410–1420
- (конференция) (2018). Neoglycolipids Micelle-like Structures as a Basis for Drug Delivery Systems. KnE Energy 3 (2), 519–527
- (2018). Effect of sorting boar spermatozoa by sex chromosomes on oviduct cell binding. Theriogenology 108, 22–28
- (2018). Rapid one-step biotinylation of biological and non-biological surfaces. Sci Rep 8 (1), 2845
- (2018). Variable Effects of Growth Factors on Developmental Gene Expression in Pancreatic Cancer Cells. Dokl Biochem Biophys 481 (1), 217–218
- (2018). Luciferase of the Japanese syllid polychaete Odontosyllis umdecimdonta. Biochem Biophys Res Commun 502 (3), 318–323
- (2017). Lectin activity of the pneumococcal pilin proteins. Sci Rep 7 (1), 17784
- (2018). Improving therapeutic potential of antibacterial spider venom peptides: coarse-grain molecular dynamics guided approach. Future Med Chem 10 (19), 2309–2322
- (2018). SypHer3s: A genetically encoded fluorescent ratiometric probe with enhanced brightness and an improved dynamic range. Chem Commun (Camb) 54 (23), 2898–2901
- (2018). Three-Dimensional Structure of Recombinant Adenine Phosphoribosyltransferase from Thermophilic Bacterial Strain Thermus thermophilus HB27. Russ. J. Bioorganic Chem. 44 (5), 504–510
- (2018). An explanation for the narrow carbohydrate substrate specificity of adenine phosphoribosyltransferase from Thermus thermophilus from the model of the enzyme, substrate and magnesium cation co-factor complex. J Biomol Struct Dyn 37 (17), 1–5
- (2018). Correlation between Expression of KLF5 and ZEB1 Transcription Factor Genes in Pancreatic Cancer. Dokl Biochem Biophys 481 (1), 219–221
- (2018). Methods of In Vivo Gene-Specific Knockdown Using Morpholino and Vivo-Morpholino Oligonucleotides. Russ. J. Bioorganic Chem. 44 (3), 358–361
- (2018). Ras-dva small GTPases lost during evolution of amniotes regulate regeneration in anamniotes. Sci Rep 8 (1), 13035
- (2018). Heterogeneous Expression of Embryonal Development Master Regulator SOX9 in Patients with Pancreatic Cancer. Dokl Biochem Biophys 481 (1), 208–211
- (2018). In Vitro Study of Antitumor Effect of Antimicrobial Peptide Tachyplesin I in Combination with Cisplatin. Bull Exp Biol Med 165 (2), 220–224
- (2018). The use of plant lectins to regulate H1N1 influenza A virus receptor binding activity. PLoS One 13 (4), e0195525
- (2018). Hostspecific glycans are correlated with susceptibility to infection by lagoviruses, but not with their virulence. J Virol 92 (4),
- (2018). Cytotoxic Potential of the Novel Horseshoe Crab Peptide Polyphemusin III. Mar Drugs 16 (12),
- (2018). Improved spot morphology for printed glycan arrays. Biotechniques 64 (3), 110–116
- (2018). Combined antibacterial effects of goat cathelicidins with different mechanisms of action. Front Microbiol 9 (NOV), 2983
- (2018). Efficient silica synthesis from tetra(glycerol)orthosilicate with cathepsin- and silicatein-like proteins. Sci Rep 8 (1), 16759
- (2018). Primary Structure Analysis of Antifungal Peptides from Cultivated and Wild Cereals. Plants (Basel) 7 (3),
- (2018). Blood Group O→A Transformation by Chemical Ligation of Erythrocytes. Chembiochem 20 (2), 131–133
- (2019). Diversity of Harpin-Like Defense Peptides from Barnyard Grass (Echinochloa crusgalli L.) Seeds. Dokl Biochem Biophys 484 (1), 6–8
- (2018). Missed Druggable Cancer Hallmark: Cancer–Stroma Symbiotic Crosstalk as Paradigm and Hypothesis for Cancer Therapy. Bioessays 40 (11), e1800079
- (2018). Crystallization and Preliminary X-ray Diffraction Study of Purine Nucleoside Phosphorylase from the Thermophilic Bacterium Thermus thermophilus Strain HB27. Cryst. Rep 63 (5), 761–764
- (2018). YesU from Bacillus subtilis preferentially binds fucosylated glycans. Sci Rep 8 (1), 13139
- (2018). Modulation of Human Complement System by Antimicrobial Peptide Arenicin-1 from Arenicola marina. Mar Drugs 16 (12), 1–11
- (2018). Thermophilic phosphoribosyltransferases Thermus thermophilus HB27 in nucleotide synthesis. Beilstein J Org Chem 2018 (14), 3098–3105
- (2018). Salicylic acid influences the protease activity and posttranslation modifications of the secreted peptides in the moss Physcomitrella patens. J Pept Sci 25 (2), e3138
- (2018). Repertoire of Abs primed by bacteria in gnotobiotic mice. Innate Immun 24 (3), 180–187
- (2018). Molecular mechanisms of the juvenile form of Batten disease: Important role of MAPK signaling pathways (ERK1/ERK2, JNK and p38) in pathogenesis of the malady. Biol Direct 13 (1), 19
- (2018). Genetic Regulatory Mechanisms of Evolution and Embryogenesis in a Distorting Mirror of Carcinogenesis. Russ J Genet 54 (2), 145–156
- (2018). Genetically encoded fluorescent indicators for live cell pH imaging. BIOCHIM BIOPHYS ACTA 1862 (12), 2924–2939
- (2018). Plant Pathogenesis-Related Proteins Binding Lipids and Other Hydrophobic Ligands. Russ. J. Bioorganic Chem. 44 (6), 586–594
- (2018). Unsolvable Problems of Biology: It Is Impossible to Create Two Identical Organisms, to Defeat Cancer, or to Map Organisms onto Their Genomes. Biochemistry (Mosc) 83 (4), 370–380
- (2018). Plant Defensins: Structure, Functions, Biosynthesis, and the Role in the Immune Response. Russ. J. Bioorganic Chem. 44 (3), 261–278
- (2015). Novel Mechanism of Bioluminescence: Oxidative Decarboxylation of a Moiety Adjacent to the Light Emitter of Fridericia Luciferin. Angew Chem Int Ed Engl 54 (24), 7065–7067
- (2015). The Chemical Basis of Fungal Bioluminescence. Angew Chem Int Ed Engl 54 (28), 8124–8128
- (2015). Pioneer transcription factors in normal development and carcinogenesis. Russ. J. Bioorganic Chem. 41 (6), 570–577
- (2015). Adult stem cells and other cancer residents. Part I. Mol. Gen. Microbiol. Virol. 30 (3), 107–113
- (2015). Preparation of growth hormone receptor GHR-(254-298) transmembrane fragments in a cell-free expression system for structural studies. Russ. J. Bioorganic Chem. 41 (6), 631–637
- (2015). GMDP: unusual physico-chemical and biological properties of the anomeriс forms. J Pept Sci 21 (9), 717–722
- (2015). Genetic markers for lung and esophagus common precursor cells in human development. Dokl Biochem Biophys 463 (1), 203–208
- (2015). PH-sensor properties of a fluorescent protein from Dendronephthya sp. Russ. J. Bioorganic Chem. 41 (6), 602–606
- (2015). The effect of mutations in the inserted domain of ATP-dependent Lon protease from E. coli on the enzyme function. Russ. J. Bioorganic Chem. 41 (5), 518–524
- (2015). Specific pools of endogenous peptides are present in gametophore, protonema, and protoplast cells of the moss Physcomitrella patens. BMC Plant Biol 15 (1), 87
- (2015). NMR Dynamics of Transmembrane and Intracellular Domains of p75NTR in Lipid-Protein Nanodiscs. Biophys J 109 (4), 772–782
- (2015). The ag1 gene is required for the fin regeneration in Danio rerio. Russ. J. Bioorganic Chem. 41 (4), 379–382
- (2015). A point mutation of the Noggin2 protein increasing its binding capacity to activin. Russ. J. Bioorganic Chem. 41 (6), 675–677
- (2015). Novel peptide chemistry in terrestrial animals: Natural luciferin analogues from the bioluminescent earthworm fridericia heliota. Chemistry 21 (10), 3942–3947
- (2015). Poised RNA polymerase II and master regulation in Metazoa. Mol Biol 49 (6), 810–817
- (2015). A novel expression cassette delivers efficient production of exclusively tetrameric human butyrylcholinesterase with improved pharmacokinetics for protection against organophosphate poisoning. Biochimie 118, 51–59
- (2015). Bovine serum albumin as a universal suppressor of non-specific peptide binding in vials prior to nano-chromatography coupled mass-spectrometry analysis. Anal Chim Acta 893, 57–64
- (2015). Specificity of human galectins on cell surfaces. Biochemistry (Mosc) 80 (7), 846–856
- (2015). Synthetic glycolipid-like constructs as tools for glycobiology research, diagnostics, and as potential therapeutics. Biochemistry (Mosc) 80 (7), 857–871