The novel fluorescent membrane probe, bis(cyclohexyl)-BODIPY (BCHB)-labeled phosphatidylcholine, is structurally similar to 1,3,5,7-tetramethyl-BODIPY (TMB)-labeled phosphatidylcholine. Formally, BCHB and TMB have similar systems of conjugated bonds; however, spectral properties of the probes are notably different. BCHB and TMB have a perfect spectral overlap. The fact makes BCHB a good FRET acceptor for TMB. Thus, the pair of phosphatidylcholines labeled with BCHB and TMB is a good tool for FRET-based membrane studies, e.g. lipid transfer studies.