Institute of
bioorganic chemistry

Scientists from the Laboratory of biosynthesis of physiologically active compounds and Laboratory of biopharmaceutical technologies (IBCH RAS) and EIMB RAS synthesized a new series of flexible 5′-norcarbocyclic aza/deaza-purine nucleoside analogs and evaluated as potential inhibitors of E. coli purine nucleoside phosphorylase. The synthesized compounds were evaluated as potential inhibitors of E. coli purine nucleoside phosphorylase. Three analogs were found to be noncompetitive inhibitors with inhibition constants of 14–24mM. From the data obtained, it can be assumed that the new 5′-norcarbocyclic nucleoside analogs interact with the active site of the PNP like natural heterocyclic bases. But at the same time the presence of a cyclopentyl moiety with 2′ and 3′ hydroxyls is necessary for the inhibitory properties, since compounds without those groups did not exhibit an inhibitory effect under the experimental conditions. The results are published in the Frontiers in Chemistry. Learn more

Scientists from the Group of molecular tools for living system studies (IBCh RAS), Department of chemistry of Natural Compounds (Faculty of Chemistry, MSU) and Osipyan Institute of Solid State Physics, together with the Laboratory of Molecular Design and Synthesis (IBCh RAS), have developed external SERS-active responsive elements based on positively charged black hole quencher (BHQ) derivatives. The proposed derivatives as external responsive elements can increase sensitivity, simplify biosensors and reduce their cost, as well as speed up the process of quantitative and qualitative analyte determination. The study results are published in Analytica Chimica Acta (IF 6.911) and Frontiers in chemistry (IF 5.545). Learn more

A new structural group of antibiotics produced by a new strain Streptomyces sp. KMM 9044 was discovered as a result of teamwork of scientists from the Institute of Bioorganic Chemistry (IBCh RAS) and the Elyakov Pacific Institute of Bioorganic Chemistry (TIBOCh RAS). During the cultivation of the strain in the marine environment, two compounds with antibacterial activity were isolated. The structure of these compounds was established using the NMR and HRMS methods and also confirmed by a series of chemical transformations. The family of new compounds are chlorinated cyclic depsiheptapeptides containing 3-hydroxy-4-chlorvaline and 4-acetoxy-5-methylproline, non-standard amino acids in the depsipeptide macrocyclic structure and a glyceric acid residue. An interesting property of these antibiotics is the strong selective inhibition of a number of Gram-positive bacteria: Micrococcus sp., Arthrobacter sp., and Mycobacterium smegmatis. The results are published in Organic Letters. Learn more

Plant lignans are active components of many herbs, which makes them the research objects for therapeutic agents development for practical use. They provide diverse naturally-occurring pharmacophores, which allows them to interact with various enzymes, receptors, ion channels and signaling molecules. A team of scientists from IBCh RAS published a review that summarizes the comprehensive knowledge about lignan use as a bioactive compounds in disorders associated with oxidative stress and inflammation, pharmacological effects in vitro and in vivo, molecular mechanisms underlying these effects, and chemical synthesis approaches. The work is published in the International Journal of Molecular Sciences. Learn more

Recently, a team of scientists from the Laboratory of molecular oncology IBCH RAS together with the colleagues from other Russian Institutes uncovered that human telomerase RNA, which was considered non-coding, has a coding potential. In human cells, there are several isoforms of the telomerase RNA transcript, and hTERC gene expression are not silenced when telomerase is inactivated. Authors hypothesized that the biogenesis of the primary transcript should determine the function of the end product of human telomerase RNA gene expression. Analyzing the influence of various factors of RNA biogenesis on the processing and transport of human telomerase RNA, scientists found that an elongated form of human telomerase RNA (mRNA encoding the previously identified hTERP protein) accumulates in the cytoplasm when mechanism of degradation of polyadenylated RNAs is inhibited. The results are published in the Biomedicines.