Chemo-enzymatic synthesis of 5-substituted ribavirin analogs: Unexpected cooperative effect in the interaction of 5-alkyloxymethyl 1,2,4-triazol-3-carboxamides with E. coli purine nucleoside phosphorylase active site
A team of scientists from the Department of Biotechnology of IBCh in collaboration with colleagues from ITHT-RTU-MIREA synthesized a series of 5-alkyl/aryl-hydroxymethyl- ribavirin analogs. Ribonucleosides were synthesized by classical chemical glycosylation, while 2'-deoxyribonucleosides were synthesized by transglycosylation with E. coli PNP. When studying the kinetic parameters, a positive cooperative effect of PNP active sites was discovered for the first time. Nucleosides were active against herpes simplex virus type 1 (HSV-1) and influenza virus H5N1. The work was published in the journal Sustainable Chemistry and Pharmacy (IF 5.464, Q2).
november 3, 2022