Nat Methods, 2024, 21(3):406-410

An improved pathway for autonomous bioluminescence imaging in eukaryotes.

The discovery of the bioluminescence pathway in the fungus Neonothopanus nambi enabled engineering of eukaryotes with self-sustained luminescence. However, the brightness of luminescence in heterologous hosts was limited by performance of the native fungal enzymes. Here we report optimized versions of the pathway that enhance bioluminescence by one to two orders of magnitude in plant, fungal and mammalian hosts, and enable longitudinal video-rate imaging.

Shakhova ES, Karataeva TA, Markina NM, Mitiouchkina T, Palkina KA, Perfilov MM, Wood MG, Hoang TT, Hall MP, Fakhranurova LI, Alekberova AE, Malyshevskaia AK, Gorbachev DA, Bugaeva EN, Pletneva LK, Babenko VV, Boldyreva DI, Gorokhovatsky AY, Balakireva AV, Gao F, Choob VV, Encell LP, Wood KV, Yampolsky IV, Sarkisyan KS, Mishin AS

Department of Biomolecular Chemistry, Laboratory of Chemistry of Metabolic Pathways, Laboratory of Molecular Bases of Plant Stress Resistance, Laboratory of optical bioimaging

: 10.1038/s41592-023-02152-y
Scopus: 2-s2.0-85182866589
: 38253843
IBCH: 11122
Ссылка на статью в журнале: https://www.nature.com/articles/s41592-023-02152-y
Кол-во цитирований на 08.2024: 4
Данные статьи проверены модераторами 2024-01-26

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