Antioxidants (Basel), 2020, 9(9):1-23

Soluble Cyanobacterial Carotenoprotein as a Robust Antioxidant Nanocarrier and Delivery Module.

To counteract oxidative stress, antioxidants including carotenoids are highly promising, yet their exploitation is drastically limited by the poor bioavailability and fast photodestruction, whereas current delivery systems are far from being efficient. Here we demonstrate that the recently discovered nanometer-sized water-soluble carotenoprotein from Anabaena sp. PCC 7120 (termed AnaCTDH) transiently interacts with liposomes to efficiently extract carotenoids via carotenoid-mediated homodimerization, yielding violet–purple protein samples. We characterize the spectroscopic properties of the obtained pigment–protein complexes and the thermodynamics of liposome–protein carotenoid transfer and demonstrate the delivery of carotenoid echinenone from AnaCTDH into liposomes with an efficiency of up to 70 ± 3%. Most importantly, we show efficient carotenoid delivery to membranes of mammalian cells, which provides protection from reactive oxygen species (ROS). Incubation of neuroblastoma cell line Tet21N in the presence of 1 μM AnaCTDH binding echinenone decreased antimycin A ROS production by 25% (p

Maksimov EG, Zamaraev AV, Parshina EY, Slonimskiy YB, Slastnikova TA, Abdrakhmanov AA, Babaev PA, Efimova SS, Ostroumova OS, Stepanov AV, Slutskaya EA, Ryabova AV, Friedrich T, Sluchanko NN

IBCH: 8782
Ссылка на статью в журнале: https://www.mdpi.com/2076-3921/9/9/869
Кол-во цитирований на 04.2024: 13
Данные статьи проверены модераторами 2020-09-21

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