New chaotropic mixtures simplify the genomic DNA preparation from fungi for PCR use
Researchers from the Laboratory of neuroreceptors and neuroregulators of IBCh RAS have improved the method of genomic DNA preparation from fungi for PCR diagnostics. The authors constructed special chaotropic mixtures for obtaining highly purified cell envelopes included DNA by high-temperature extraction from yeast biomass. Chaotropic mixture consisted from 7M urea, 1% SDS, 100 mM ammonia, 25 mM sodium citrate shown best effectiveness. This mixture provided almost complete removal of RNA and lipids, as well as most of the proteins, followed single-stage production of DNA-containing cell envelopes suitable for PCR. The approach developed in this study is promising for PCR diagnostics and taxonomic studies of yeast and other microorganism having stable cell wall. The study is published in the journal Analytica Chimica Acta. Learn more
26 июля 2023 года