Red light-emitting short Mango-based system enables tracking a mycobacterial small noncoding RNA in infected macrophages
Scientists from IBCH RAS, together with colleagues from the Federal Research Center for Physical and Chemical Medicine and other Russian institutes, have developed a platform for fluorescent imaging of small non-coding RNAs (sncRNAs) in living cells. The system consists of a fluorogenic cyanine dye and a genetically encoded Mango II aptamer-based label, which is attached to the sncRNA of interest and causes an increase in dye fluorescence upon complex formation. An approach to optimizing the spectral properties of dyes was proposed and a minimal genetically encoded label (52 nt) for sncRNA visualization was developed. The applicability of the system for intracellular imaging and tracking of sncRNA was verified using an intracellular internalization of Mycobacterium smegmatis by a host macrophage. The work was published in Nucleic Acids Research.
26 февраля 2023 года