Proc Natl Acad Sci U S A, 2022, 119(15):e2114905119

Persistence of plasmids targeted by CRISPR interference in bacterial populations

SignificanceRNA-guided CRISPR-Cas nucleases efficiently protect bacterial cells from phage infection and plasmid transformation. Yet, the efficiency of CRISPR-Cas defense is not absolute. Mutations in either CRISPR-Cas components of the host or mobile genetic elements regions targeted by CRISPR-Cas inactivate the defensive action. Here, we show that even at conditions of active CRISPR-Cas and unaltered targeted plasmids, a kinetic equilibrium between CRISPR-Cas nucleases action and plasmid replication processes allows for existence of a small subpopulation of plasmid-bearing cells on the background of cells that have been cured from the plasmid. In nature, the observed diversification of phenotypes may allow rapid changes in the population structure to meet the demands of the environment.

Mamontov V, Martynov A, Morozova N, Bukatin A, Staroverov DB, Lukyanov KA, Ispolatov Y, Semenova E, Severinov K

IBCH: 9973
Ссылка на статью в журнале: https://pnas.org/doi/full/10.1073/pnas.2114905119
Кол-во цитирований на 07.2024: 2
Данные статьи проверены модераторами 2022-05-04

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