Sci Rep, 2019, 9(1):413

Specific refolding pathway of viscumin A chain in membrane-like medium reveals a possible mechanism of toxin entry into cell

How is a water-soluble globular protein able to spontaneously cross a cellular membrane? It is commonly accepted that it undergoes significant structural rearrangements on the lipid-water interface, thus acquiring membrane binding and penetration ability. In this study molecular dynamics (MD) simulations have been used to explore large-scale conformational changes of the globular viscumin A chain in a complex environment – comprising urea and chloroform/methanol (CHCl3/MeOH) mixture. Being well-packed in aqueous solution, viscumin A undergoes global structural rearrangements in both organic media. In urea, the protein is “swelling” and gradually loses its long-distance contacts, thus resembling the “molten globule” state. In CHCl3/MeOH, viscumin A is in effect turned “inside out”. This is accompanied with strengthening of the secondary structure and surface exposure of hydrophobic epitopes originally buried inside the globule. Resulting solvent-adapted models were further subjected to Monte Carlo simulations with an implicit hydrophobic slab membrane. In contrast to only a few point surface contacts in water and two short regions with weak protein-lipid interactions in urea, MD-derived structures in CHCl3/MeOH reveal multiple determinants of membrane interaction. Consequently it is now possible to propose a specific pathway for the structural adaptation of viscumin A with respect to the cell membrane – a probable first step of its translocation into cytoplasmic targets.

Volynsky PE, Nolde DE, Zakharova GS, Palmer RA, Tonevitsky AG, Efremov RG

IBCH: 7425
Ссылка на статью в журнале: http://www.nature.com/articles/s41598-018-36310-6
Кол-во цитирований на 06.2024: 3
Данные статьи проверены модераторами 2019-01-23

Список научных проектов, где отмечена публикация

  1. -Молекулярно-биофизические аспекты олигомеризации мембранных доменов рецепторов, определяющие клеточную сигнализацию в норме и онкогенезе (January 6, 2018 — December 31, 2022). Efremov R.G.. Grant, RSF.