Dokl Biochem Biophys, 2023, 508(1):17-20

Barnase-barstar Specific Interaction Regulates Car-T Cells Cytotoxic Activity toward Malignancy

The development of CAR-T specific therapy made a revolution in modern oncology. Despite the pronounced therapeutic effects, this novel approach displayed several crucial limitations caused by the complications in pharmacokinetics and pharmacodynamics controls. The presence of the several severe medical complications of CAR-T therapy initiated a set of attempts aimed to regulate their activity in vivo. We propose to apply the barnase-barstar system to control the cytotoxic antitumor activity of CAR-T cells. To menage the regulation targeting effect of the system we propose to use barstar-modified CAR-T cells together with barnase-based molecules. Barnase was fused with designed ankyrin repeat proteins (DARPins) specific to tumor antigens HER2 (human epidermal growth factor receptor 2) The application of the system demonstrates the pronounced regulatory effects of CAR-T targeting.

Kalinin RS, Shipunova VO, Rubtsov YP, Ukrainskay VM, Schulga A, Konovalova EV, Volkov DV, Yaroshevich IA, Moysenovich AM, Belogurov AA, Telegin GB, Chernov AS, Maschan MA, Terekhov SS, Knorre VD, Khurs E, Gnuchev NV, Gabibov AG, Deyev SM

Department of Peptide and Protein Technologies, Laboratory of biocatalysis, Laboratory of hormonal regulation proteins, Laboratory of proteolytic enzyme chemistry, Laboratory of antibiotic resistance, Department of immunology, Laboratory of molecular immunology, Department of Molecular Neurobiology, Laboratory of neuroreceptors and neuroregulators, Department of functioning of living systems, Laboratory of molecular virology, Laboratory of bioinformatics approaches in combinatorial chemistry and biology, Department of biotechnology, Laboratory of Instruments for Diagnosis and Therapy of Infectious Diseases, Nursery for laboratory animals, Veterinary Service

: 10.1134/S1607672922700041
Scopus: 2-s2.0-85147286886
: 36653580
IBCH: 10477
Ссылка на статью в журнале: https://link.springer.com/10.1134/S1607672922700041
Кол-во цитирований на 10.2024: 3
Данные статьи проверены модераторами 2023-01-20

Список научных проектов, где отмечена публикация

  1. -Структурные и кинетические особенности презентации антигенов как ключ к пониманию механизмов индукции аутоиммунных патологий и лимфомогенезиса (July 1, 2017 — June 30, 2023). Gabibov A.G.. Grant, RSF.