In this study, we first used prestin, an electromotive protein of the mammalian auditory analyzer, as a voltage-sensitive core of a genetically encoded voltage indicator. Prestin-5, one of the chimeric proteins obtained as a fusion of prestin and the fluorescent reporter FusionRed, demonstrated a fluorescent response to a change in membrane potential, characterized by submillisecond kinetics. Thus, we demonstrate the performance of prestin in fluorescent voltage-sensitive constructs.