In autonomous luminescence technologies, the substrate for the light-emitting reaction is produced by a cascade of biochemical reactions within the cells. Because of this, measurements do not require the exogenous addition of a substrate, only light detection equipment is needed. The development of autonomous luminescence technologies over the past few years suggests that they will become the platform for a whole generation of new methods for non-invasive imaging of physiological events. The ability to genetically encode the entire cascade of reactions required for luminescence opens up the possibility of non-invasively imaging virtually any molecular event at the tissue level. For example, it becomes possible by placing the genes responsible for luminescence under the control of a promoter of interest to monitor the activity of this promoter in the tissues throughout an organism's life. And by distributing luminescence genes between the pathogen and the host organism, one can receive a signal only from infected cells, and visualize the progress of the infection in real time. Our scientific team has made significant progress in developing such technologies in recent years (Mitiouchkina et al. Nature Biotechnology 2020, Shakhova et al. Nature Methods 2024). But although we were able to achieve high luminescence brightness in the tissues of plants and fungi, in animal cells the biosynthesis of the bioluminescent substrate occurs ineffectively, leading to extremely low luminescence brightness. This limitation does not yet allow the use of autonomous luminescence in biomedical applications. In this project, our goal is to create a technology for autonomous luminescence that provides sufficient brightness in animal cells to visualize physiology at the tissue level.