Int J Mol Sci, 2020, 21(22):1-12

Genetically encoded red photosensitizers with enhanced phototoxicity

Licensee MDPI, Basel, Switzerland. Genetically encoded photosensitizers are increasingly used as optogenetic tools to control cell fate or trigger intracellular processes. A monomeric red fluorescent protein called SuperNova has been recently developed, however, it demonstrates suboptimal characteristics in most phototoxicity‐based applications. Here, we applied directed evolution to this protein and identified SuperNova2, a protein with S10R substitution that results in enhanced brightness, chromophore maturation and phototoxicity in bacterial and mammalian cell cultures.

IBCH: 8912
Ссылка на статью в журнале: https://www.mdpi.com/1422-0067/21/22/8800
Кол-во цитирований на 07.2024: 8
Данные статьи проверены модераторами 2020-11-26

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