Biochimie, 2015, 118:116-122

A new anticancer toxin based on HER2/neu-specific DARPin and photoactive flavoprotein miniSOG

V. and Société Française de Biochimie et Biologie Moléculaire (SFBBM). All rights reserved. Cytotoxic effects of a new targeted phototoxin DARPin-miniSOG and mechanism of its action were investigated in vitro. It was determined that DARPin-miniSOG causes light-induced death of HER2/neu-positive cancer cells (IC50 0.8 μM). Treatment of the cells with DARPin-miniSOG in the presence of ascorbic acid eliminated the light-induced cytotoxic action of the protein. This observation suggests the involvement of oxidative stress in the mechanism of the phototoxin action. DNA fragmentation analysis, caspase-3 activity assay and PI-staining of HER2/neu-positive cancer cells treated with DARPin-miniSOG indicated that phototoxin induces necrotic cell death under blue light illumination. Co-localization analysis showed that DARPin-miniSOG accumulates mostly in endosomes and lysosomes.

IBCH: 3780
Ссылка на статью в журнале: https://linkinghub.elsevier.com/retrieve/pii/S0300908415002722
Кол-во цитирований на 07.2024: 42
Информация пока не проверена модераторами

Список научных проектов, где отмечена публикация

  1. -Комплексный подход к биоинженерии мультифункциональных соединений направленного действия для диагностики и терапии рака (January 6, 2014 — December 31, 2018). Deyev S.M.. Grant, RSF.