Nat Methods, 2018, 15(8):601-604

Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy

Reversibly photoswitchable fluorescent proteins (rsFPs) are gaining popularity as tags for optical nanoscopy because they make it possible to image with lower light doses. However, green rsFPs need violet-blue light for photoswitching, which is potentially phototoxic and highly scattering. We developed new rsFPs based on FusionRed that are reversibly photoswitchable with green-orange light. The rsFusionReds are bright and exhibit rapid photoswitching, thereby enabling nanoscale imaging of living cells.

Pennacchietti F, Serebrovskaya EO, Faro AR, Shemyakina II, Bozhanova NG, Kotlobay AA, Gurskaya NG, Bodén A, Dreier J, Chudakov DM, Lukyanov KA, Verkhusha VV, Mishin AS, Testa I

Laboratory of Chemistry of Metabolic Pathways, Laboratory of immunosequencing methods, Laboratory of genetically encoded molecular tools, Laboratory of optical bioimaging

: 10.1038/s41592-018-0052-9
Scopus: 2-s2.0-85049608980
: 29988095
IBCH: 2997
Ссылка на статью в журнале: http://www.nature.com/articles/s41592-018-0052-9
Кол-во цитирований на 11.2024: 70
Данные статьи проверены модераторами 2018-08-01

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