Mechanical forces arising in developing embryos are able to spread over considerable distances inembryos and affect gene expression. The study of mechano-dependent transcription is one of the topicalissues of modern developmental biology. One of the approaches to solving this problem is the use of lowmolecular-weight myosin inhibitors. In this paper, we studied by RT-PCR the effect of four myosin inhibitors—blebbistatin, S-nitroblebbistatin, ML-9 and 2,3-butanedione monoxime—on the expression of eightmechano-dependent genes in early embryos of the clawed frog. When exposed to myosin inhibitors, weobserved a change in the expression level of four mechano-dependent genes, namely gsc, myh6, Xbra, andxmc. These results indicate that mechanical forces can play a role in the anterio-posterior patterning of theembryo by regulating the expression of the corresponding genes10.1134/S1068162022040094