RUSSIAN ACADEMY OF SCIENCES
Shemyakin & Ovchinikov Institute of Bioorganic Chemistry
Laboratory of Molecular Technologies
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General information
Professional appointments
Education
Scientific background
Publications

Arkady F. Fradkov

GENERAL INFORMATION
Born: June 1, 1967, Tula, Russia (USSR)
Citizenship: Russia
Business address:
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Micklukho-Maklaya 16/10, 117871, Moscow, Russia;
tel: 7(495) 429-80-20
fax: 7(495) 330-70-56
e-mail: arc@ibch.ru

PROFESSIONAL APPOINTMENTS
Senior scientist, Laboratory of Molecular Technologies (prev. Laboratory of Genes for Regeneration), Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.

EDUCATION
1992 M.Sc. Biotechnology, Moscow Institute of Fine Chemical Technology, Moscow, Russia.
M.Sc. thesis: "Construction of vector pVEV for gene expression in E.coli". The work was fulfilled in Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia. Laboratory of Gene Bioengineering headed by Prof. V.A. Efimov.

SCIENTIFIC BACKGROUND
1990-1992 Research assistant. Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia. Laboratory of Gene Bioengineering headed by Prof. V.A. Efimov.
1992-1995 postgraduate student, Laboratory of Gene Bioengineering, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.

Area of expertise: molecular cloning, DNA sequencing, construction of the plasmid vectors for cloning and expression of various eucariotic genes in bacteria, Northern and Western analyses.
Ph.D. thesis: “Investigation of approaches to production of the recombinant proteins as hybrids with streptavidin and metal-binding peptides” - 1995. Supervisor - Prof. V.A. Efimov.
1995-1996 junior scientist, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia. Laboratory of Structure and Function of Human Genes headed by Prof. E.D.Sverdlov.
1996-1998 Research scientist, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia. Laboratory of Structure and Function of Human Genes headed by Prof. E.D.Sverdlov.
1998- 1999 Research scientist, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia. Laboratory of Genes for Regeneration headed by S.A.Lukyanov, Ph.D.
1999- present Senior research scientist, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia. Laboratory of Molecular Technologies (prev. Laboratory for Genes for Regeneration) headed by S.A.Lukyanov, Ph.D.
Area of expertise: gene expression in bacterial systems, PCR technology, subtractive hybridisation, differential display technique, RACE, cDNA cloning and analysis.


PUBLICATIONS
  1. Shcherbo D, Merzlyak EM, Chepurnykh TV, Fradkov AF, Ermakova GV, Solovieva EA, Lukyanov KA, Bogdanova EA, Zaraisky AG, Lukyanov S, Chudakov DM.
    Bright far-red fluorescent protein for whole-body imaging.
    Nat Methods. 2007 Sep;4(9):741-6
    PMID: 17721542

  2. Merzlyak EM, Goedhart J, Shcherbo D, Bulina ME, Shcheglov AS, Fradkov AF, Gaintzeva A, Lukyanov KA, Lukyanov S, Gadella TW, Chudakov DM.
    Bright monomeric red fluorescent protein with an extended fluorescence lifetime.
    Nat Methods. 2007 Jul;4(7):555-7
    PMID: 17572680

  3. Lukyanov KA, Chudakov DM, Fradkov AF, Labas YA, Matz MV, Lukyanov S.
    Discovery and properties of GFP-like proteins from nonbioluminescent anthozoa.
    Methods Biochem Anal. 2006;47:121-38
    PMID: 16335712

  4. Gurskaya NG, Verkhusha VV, Shcheglov AS, Staroverov DB, Chepurnykh TV, Fradkov AF, Lukyanov S, Lukyanov KA.
    Engineering of a monomeric green-to-red photoactivatable fluorescent protein induced by blue light.
    Nat Biotechnol. 2006 Apr;24(4):461-5
    PMID: 16550175

  5. Belousov VV, Fradkov AF, Lukyanov KA, Staroverov DB, Shakhbazov KS, Terskikh AV, Lukyanov S.
    Genetically encoded fluorescent indicator for intracellular hydrogen peroxide.
    Nat Methods. 2006 Apr;3(4):281-6
    PMID: 16554833

  6. Lukyanov KA, Chudakov DM, Fradkov AF, Labas YA, Matz MV and Lukyanov SA.
    Discovery and properties of GFP-like proteins from non-bioluminescent Anthozoa.
    In: Green fluorescent protein: properties and applications. Chalfie, M., Kain, S., (Eds). Willey-Liss, New York, 2005; 121-38

  7. Yanushevich YG, Shagin DA, Fradkov AF, Shakhbazov KS, Barsova EV, Gurskaya NG, Labas YA, Matz MV, Lukyanov KA, Lukyanov SA.
    Spectral diversity among the members of the family of Green Fluorescent Protein in hydroid jellyfish (Cnidaria, Hydrozoa).
    Bioorg Khim. 2005 Jan-Feb;31(1):49-53 (in Russian)
    PMID: 15787213

  8. Shagin DA, Barsova EV, Yanushevich YG, Fradkov AF, Lukyanov KA, Labas YA, Semenova TN, Ugalde JA, Meyers A, Nunez JM, Widder EA, Lukyanov SA, Matz MV.
    GFP-like proteins as ubiquitous Metazoan superfamily: evolution of functional features and structural complexity.
    Mol Biol Evol. 2004 May;21(5):841-50
    PMID: 14963095

  9. Gurskaya NG, Fradkov AF, Pounkova NI, Staroverov DB, Bulina ME, Yanushevich YG, Labas YA, Lukyanov S, Lukyanov KA.
    A colourless green fluorescent protein homologue from the non-fluorescent hydromedusa Aequorea coerulescens and its fluorescent mutants.
    Biochem J. 2003 Jul 15;373(Pt 2):403-8
    PMID: 12693991

  10. Fradkov AF, Verkhusha VV, Staroverov DB, Bulina ME, Yanushevich YG, Martynov VI, Lukyanov S, Lukyanov KA.
    Far-red fluorescent tag for protein labelling.
    Biochem J. 2002 Nov 15;368(Pt 1):17-21
    PMID: 12350221

  11. Labas YA, Gurskaya NG, Yanushevich YG, Fradkov AF, Lukyanov KA, Lukyanov SA, Matz MV.
    Diversity and evolution of the green fluorescent protein family.
    Proc Natl Acad Sci U S A. 2002 Apr 2;99(7):4256-61
    PMID: 11929996

  12. Terskikh AV, Fradkov AF, Zaraisky AG, Kajava AV, Angres B.
    Analysis of DsRed Mutants. Space around the fluorophore accelerates fluorescence development.
    J Biol Chem. 2002 Mar 8;277(10):7633-6
    PMID: 11773062

  13. Yanushevich YG, Staroverov DB, Savitsky AP, Fradkov AF, Gurskaya NG, Bulina ME, Lukyanov KA, Lukyanov SA.
    A strategy for the generation of non-aggregating mutants of Anthozoa fluorescent proteins.
    FEBS Lett. 2002 Jan 30;511(1-3):11-4
    PMID: 11821040

  14. Gurskaya NG, Fradkov AF, Terskikh A, Matz MV, Labas YA, Martynov VI, Yanushevich YG, Lukyanov KA, Lukyanov SA.
    GFP-like chromoproteins as a source of far-red fluorescent proteins.
    FEBS Lett. 2001 Oct 19;507(1):16-20
    PMID: 11682051

  15. Terskikh A, Fradkov A, Ermakova G, Zaraisky A, Tan P, Kajava AV, Zhao X, Lukyanov S, Matz M, Kim S, Weissman I, Siebert P.
    "Fluorescent timer": protein that changes color with time.
    Science. 2000 Nov 24;290(5496):1585-8
    PMID: 11090358

  16. Lukyanov KA, Fradkov AF, Gurskaya NG, Matz MV, Labas YA, Savitsky AP, Markelov ML, Zaraisky AG, Zhao X, Fang Y, Tan W, Lukyanov SA.
    Natural animal coloration can be determined by a nonfluorescent green fluorescent protein homolog.
    J Biol Chem. 2000 Aug 25;275(34):25879-82
    PMID: 10852900

  17. Fradkov AF, Chen Y, Ding L, Barsova EV, Matz MV, Lukyanov SA.
    Novel fluorescent protein from Discosoma coral and its mutants possesses a unique far-red fluorescence.
    FEBS Lett. 2000 Aug 18;479(3):127-30
    PMID: 10981720

  18. Tarabykin V, Britanova O, Fradkov A, Voss A, Katz LS, Lukyanov S, Gruss P.
    Expression of PTTG and prc1 genes during telencephalic neurogenesis.
    Mech Dev. 2000 Apr;92(2):301-4
    PMID: 10727870

  19. Bogush ML, Velikodvorskaya TV, Lebedev YB, Nikolaev LG, Lukyanov SA, Fradkov AF, Pliyev BK, Boichenko MN, Usatova GN, Vorobiev AA, Andersen GL, Sverdlov ED.
    Identification and localization of differences between Escherichia coli and Salmonella typhimurium genomes by suppressive subtractive hybridization.
    Mol Gen Genet. 1999 Dec;262(4-5):721-9
    PMID: 10628854

  20. Matz MV, Fradkov AF, Labas YA, Savitsky AP, Zaraisky AG, Markelov ML, Lukyanov SA.
    Fluorescent proteins from nonbioluminescent Anthozoa species.
    Nat Biotechnol. 1999 Oct;17(10):969-73
    PMID: 10504696

  21. Matz MV, Shagin DA, Usman NIu, Bogdanova EA, Fradkov AF, Soboleva TA, Lukyanov SA.
    Cloning of region-specific genetic markers of planaria using a new method--ordered differential display.
    Bioorg Khim. 1998 Dec;24(12):910-5 (in Russian)
    PMID: 10079949

  22. Akopyants NS, Fradkov A, Diatchenko L, Hill JE, Siebert PD, Lukyanov SA, Sverdlov ED, Berg DE.
    PCR-based subtractive hybridization and differences in gene content among strains of Helicobacter pylori.
    Proc Natl Acad Sci U S A. 1998 Oct 27;95(22):13108-13
    PMID: 9789049

  23. Fradkov AF, Lukyanov KA, Matz MV, Diatchenko LB, Siebert PD, Lukyanov SA.
    Sequence-independent method for in vitro generation of nested deletions for sequencing large DNA fragments.
    Anal Biochem. 1998 Apr 10;258(1):138-41
    PMID: 9527860

  24. Fradkov A, Berezhnoy S, Barsova E, Zavalova L, Lukyanov S, Baskova I, Sverdlov ED.
    Enzyme from the medicinal leech (Hirudo medicinalis) that specifically splits endo-epsilon(-gamma-Glu)-Lys isopeptide bonds: cDNA cloning and protein primary structure.
    FEBS Lett. 1996 Jul 22;390(2):145-8
    PMID: 8706846

  25. Efimov VA, Buriakova AA, Fradkov AF, Chakhmakhcheva OG.
    Biosynthesis of human calcitonin and mini-proinsulin in bacterial cells in the form of hybrid proteins with corresponding antisense peptides and a metal-binding peptide.
    Bioorg Khim. 1996 Jul;22(7):503-9 (in Russian)
    PMID: 8992955

  26. Efimov VA, Kalinkina AL, Fradkov AF, Chakhmakhcheva OG.
    An artificial gene, biosynthesis and properties of the recombinant Fc fragment of human immunoglobulin G1.
    Bioorg Khim. 1996 Mar;22(3):168-7 (in Russian)
    PMID: 8687508

  27. Smirnov IuV, Fradkov AF, Chakhmakhcheva OG, Efimov VA.
    Synthesis of a highly active recombinant Thermus aquaticus His6-DNA-polymerase in Escherichia coli cells and a rapid method of purifying it.
    Bioorg Khim. 1995 May;21(5):396-8 (in Russian)
    PMID: 7544973

  28. Efimov VA, Fradkov AA, Kalinkina AL, Chakhmakhcheva OG.
    Expression vectors enabling synthesis of recombinant proteins as hybrids with metal-binding peptides.
    Bioorg Khim. 1995 Jan;21(1):9-16 (in Russian)
    PMID: 7710427

  29. Efimov VA, Aronova EA, Buriakova AA, Kalinkina AL, Letunova AB, Fradkov AF, Chakhmakhcheva OG.
    Synthesis and cloning of genes for the antisense peptides for human calcitonin and miniproinsulin.
    Bioorg Khim. 1994 Jul;20(7):759-7 (in Russian)
    PMID: 7993380

  30. Efimov VA, Fradkov AF, Raskind AB, Khristin MS, Klimov VV, Chakhmakhcheva OG.
    Expression of the barley psbA gene in Escherichia coli yields a functional in vitro photosystem II protein D1.
    FEBS Lett. 1994 Jul 11;348(2):1
    PMID: 8034031

  31. Efimov VA, Reverdatto SV, Beilinson BA, Fradkov AF, Chakhmakhcheva OG.
    Expression of the gene coding for the D1-protein of barley photosystem II in Escherichia coli.
    Bioorg Khim. 1994 May;20(5):524-3 (in Russian)
    PMID: 8053946

  32. Reverdatto SV, Beilinson VA, Fradkov AF, Polushin NN, Efimov VA.
    Plasmid vectors for 'unclonable' DNA constructions.
    Nucleic Acids Symp Ser. 1991;(24):306-7
    PMID: 1841362