Effect of the protein corona of antitumor liposomes decorated with the Sialyl Lewis X selectin ligand on interactions with endothelial cells in microvascular biomodeling
Scientists of the Department of Chemical Biology of Glycans and Lipids, together with colleagues from the Institute of General Pathology and Pathophysiology of the Russian Academy of Sciences, N.F. Gamaleya Research Institute and V.N. Orekhovich Institute of Biomedical Chemistry, investigated the effect of the protein corona of targeted liposomes on internalization by endothelial cells in the conditions of modeling microvessels in a microfluidic device. The ability of liposomes decorated with a tetrasaccharide selectin ligand SiaLe X to target activated endotheliocytes was preserved under microflow conditions. However, in the human serum flow, the level of liposome uptake by cells decreased. Proteomic analysis and immunoblotting of liposome-protein complexes revealed a correlation between the content of SiaLe X and some apolipoproteins, including likely nonspecific competitors of selectins. The results are published in Pharmaceutics.
july 5, 2023