Comparative Analysis of Enzymatic Transglycosylation Using E.coli Nucleoside Phosphorylases: A Synthetic Concept for the Preparation of Purine Modified 2′-Deoxyribonucleosides from Ribonucleosides
The team of scientists from the Laboratory of Biopharmaceutical Technologies of the IBCh RAS and from the Engelhardt Institute of Molecular Biology (IMB RAS) has developed an efficient method for the preparative synthesis of modified purine 2'-deoxyribonucleosides. For this purpose, a comparative analysis of the conditions of the transglycosylation reaction catalyzed by purine (PNP) and thymidine (TP) nucleoside phosphorylases leading to the formation of 2'-deoxynucleosides, was carried out. It was shown that maximal yields of 2′-deoxyribonucleosides, especially modified, can be achieved under small excess of glycosyl-donor (7-methyl-2'-deoxyguanosine, thymidine) and a 4-fold lack of phosphate. A less than equimolar phosphate concentration allows using only a small excess of the nucleoside-donor of the carbohydrate residue to increase the yield of the reaction. The work was published in the International Journal of Molecular Sciences (IF 5.924).
june 21, 2022